7 resultados para multiplex PCR

em Universidad Politécnica de Madrid


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Although 3DTV has led the evolution of television market, its delivery by broadcast networks is still small. Now, 3DTV transmis-sions are usually done by combining both views into one common frame (side by side) to be able to use standard HDTV transmission equipment. Today, orthogonal subsampling is mostly used, but other alternatives will appear soon. Here, different subsampling schemes for both progressive and interlaced 3DTV are considered. For each possible scheme, its pre-served frequency content is analyzed and a simple interpolation filter is designed. The analysis is carried out for progressive and interlaced video and the designed filters are applied on different sequences, showing the advantages and disadvantages of the different options

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This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya) and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp) was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers. AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were the most similar, in spite of their morphological diversity.

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The correct assignment of high molecular weight glutenin subunit variants is a key task in wheat breeding. However, the traditional analysis by protein electrophoresis is sometimes difficult and not very precise. This work describes a novel DNA marker for the accurate discrimination between the Glu-B1 locus subunits Bx7 and Bx7*. The analysis of one hundred and forty two bread wheat cultivars from different countries has highlighted a great number of misclassifications in the literature that could lead to wrong conclusions in studies of the relationship between glutenin composition and wheat quality.

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The construction cost estimation systems in Spain are undeveloped and, hence, infrequently used by technicians and professionals in the building sector. However, estimation of an approximate real cost prior to the execution of the work is compulsory under current legal regulations (Technical Building Code). Therefore, the development of research projects on construction cost estimation models such as the one described and demonstrated in this talk is extremely interesting.

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The addition of preservatives to some kind of foods may be a potential risk of spoilage, as it is the transformation of sorbate into the off-odour 1-3-pentadiene by certain microbial species. This is the case of the capacity of some strains of moulds and yeasts that are able to decarboxylate sorbic acid and transform it into 1-3 pentadiene, a volatile compound with an unpleasant petroleum odour. (Casas et al. 2004).

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We extend the concept of eigenvector centrality to multiplex networks, and introduce several alternative parameters that quantify the importance of nodes in a multi-layered networked system, including the definition of vectorial-type centralities. In addition, we rigorously show that, under reasonable conditions, such centrality measures exist and are unique. Computer experiments and simulations demonstrate that the proposed measures provide substantially different results when applied to the same multiplex structure, and highlight the non-trivial relationships between the different measures of centrality introduced.

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Este proyecto versa sobre un modelo de evaluación de calidad de imagen aplicado a la optimización del ancho de banda del multiplex de televisión, mediante la realización de ensayos con distintas configuraciones de cabecera. Dicho modelo se basa en las medidas PQR y DMOS de Tektronix, destinadas a medir la percepción de las diferencias entre una secuencia antes y después de sufrir un procesado digital. Dado que actualmente, el modo de trabajo de una cabecera de televisión digital, es la multiplexación estadística (consistente en la codificación de diferentes servicios de vídeo con anchos de banda variables en función de la complejidad de las señales), las medidas estarán enfocadas a sacar conclusiones acerca de la cantidad de canales, complejidad de contenidos, y organización de los mismos en el ancho de banda disponible para emitir, manteniendo niveles de calidad Broadcast. Las medidas serán aplicadas en el proyecto para comparar el rendimiento de dos modelos de cabecera. En primer lugar serán configuradas en régimen binario constante, comparando el rendimiento de los codificadores en el área de trabajo habitual. Posteriormente se configuraran en régimen binario variable probando múltiples configuraciones, con el objetivo dar con el modelo y configuración óptima para su posterior implementación. ABSTRACT. This project concerns a picture quality assessment model applied to television multiplex bandwidth optimization by conducting test with different headend settings. This model is based on the PQR and DMOS Tektronix measures, designed to measure the differences between a sequence before and after a digital processing. Given that nowadays the working way of television headend is by statistical multiplexing (based on coding the different video services with variable bitrate depending on the complexity of the signals) measures will be focused to reach conclusions about the number of channels, complexity of content, and the way to organize them through the available bandwidth, keeping broadcast quality ratios. The measures will be applied comparing the performance of two headend models. First of all encoders will be set on constant bitrate, comparing the performance through the working bandwidth. Later on, both models will be set on variable bitrate testing multiple configurations, in order to find the optimal model/configuration for later implementation.